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KMID : 0352720190430020236
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Journal of Ginseng Research
2019 Volume.43 No. 2 p.236 ~ p.241
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Inhibitory effects of thromboxane A2 generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A2 phosphorylation and arachidonic acid release
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Shin Jung-Hae
Kwon Hyuk-Woo
Rhee Man-Hee
Park Hwa-Jin
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Abstract
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Background: Thromboxane A2 (TXA2) induces platelet aggregation and promotes thrombus formation. Although ginsenoside Ro (G-Ro) from Panax ginseng is known to exhibit a Ca2+-antagonistic antiplatelet effect, whether it inhibits Ca2+-dependent cytosolic phospholipase A2 (cPLA2α) activity to prevent the release of arachidonic acid (AA), a TXA2 precursor, is unknown. In this study, we attempted to identify the mechanism underlying G-Ro-mediated TXA2 inhibition.
Methods: We investigated whether G-Ro attenuates TXA2 production and its associated molecules, such as cyclooxygenase-1 (COX-1), TXA2 synthase (TXAS), cPLA2α, mitogen-activated protein kinases, and AA. To assay COX-1 and TXAS, we used microsomal fraction of platelets.
Results: G-Ro reduced TXA2 production by inhibiting AA release. It acted by decreasing the phosphorylation of cPLA2α, p38-mitogen-activated protein kinase, and c-Jun N-terminal kinase1, rather than by inhibiting COX-1 and TXAS in thrombin-activated human platelets.
Conclusion: G-Ro inhibits AA release to attenuate TXA2 production, which may counteract TXA2-associated thrombosis.
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KEYWORD
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Arachidonic acid, Cytosolic phospholipase A2α, Ginsenoside Ro, Mitogen-activated protein kinases, Thromboxane A2
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